目的 初步观察电针足三里穴对不全肠梗阻大鼠小肠Cajal间质细胞（interstitial cells of Cajal，ICC）数量变化的影响，为进一步探讨电针足三里穴对ICC表型变化的影响奠定基础。
方法 采用圈套法造成不全肠梗阻从而建立ICC数目减少的SD大鼠模型。取20只雌性大鼠采用简单随机法均分成正常对照组、不全肠梗阻30d未电针足三里穴组（梗阻组）、不全肠梗组30d电针足三里穴组（足三里组）和不全肠梗阻30d电针阴陵泉穴组（阴陵泉组） 4组。其中足三里组和阴陵泉组分别连续电针足三里穴或阴陵泉穴7d后，取小肠组织采用免疫组化方法以及免疫荧光观察ICC数量的变化。
结果 正常对照组、足三里组、梗阻组及阴陵泉组ICC阳性面积分别为（102 051.00±16 969.38） μm2、（92 642.12±14 854.49） μm2、（45 221.33±6 230.20） μm2和（63 136.16±8 863.91） μm2，各组间差异有统计学意义（F=21.240，P＜0.001），其中足三里组的ICC阳性面积较梗阻组高（P＜0.05）。
结论 电针足三里穴可使不全肠梗阻大鼠小肠受损的ICC数量得到部分恢复，但其具体机理有待进一步研究。
Citation：
朱晓艳,李昆,彭梅芳,王超,李运明,张国虎,王培红,王永华,汤礼军,张林. Immunohistochemical Observation of Effects of Electroacupuncture at Zusanli on Plasticity of Interstitial Cells of Cajal in Incomplete Small-Bowel Obstruction Rat. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2012, 19(9): 984-987. doi:
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Beyder A， Farrugia G. Targeting ion channels for the treatment of gastrointestinal motility disorders[J]. Therap Adv Gastroenterol， 2012， 5(1)：5-21.
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Huizinga JD， Thuneberg L， Kluppel M， et al. W/kit generequired for interstitial cells of Cajal and for intestinal pacemaker activity[J]. Nature， 1995， 373(6512)： 347-349.
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Chang IY， Glasgow NJ， TakayamaⅠ， et al. Loss of interstitial cells of Cajal and development of electrical dysfunction in murine small bowel obstruction[J]. J Physiol， 2001， 536(2)：555-568.
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Sanders KM. Interstitial cells of Cajal at the clinical and scientific interface[J]. J Physiol， 2006， 576(3)：683-687.
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Won KJ， Suzuki T， Hori M， et al. Motility disorder in experimentally obstructed intestine：relationship between muscularis inflammation and disruption of the ICC network[J]. Neurogastroenterol Motil， 2006， 18(1)：53-61.
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Yanagida H， Sanders KM， Ward SM. Inactivation of inducible nitric oxide synthase protects intestinal pacemaker cells from postoperative damage[J]. J Physiol， 2007， 582(Pt 2)：755-765.
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Yanagida H， Yanase H， Sanders KM. Intestinal surgical resection disrupts electrical rhythmicity， neural responses， and interstitial cells networks[J]. Gastroenterology， 2004， 127(6)：1748-1759.
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Farrugia G. Interstitial cells of Cajal in health and disease[J]. Neurogastroenterol Motil， 2008， 20(Suppl 1)：54-63.
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- 1. Torihashi S， Fujimoto T， Trost C， et al. Calcium oscillation linked to pacemaking of interstitial cells of Cajal[J]. J Biol Chen， 2002， 277(21)：19191-19197.
- 2. Sanders KM， Ordog T， Ward SM， et al. Physiology and pathophysiology of the interstitial cells of Cajal：from bench to bedside. Ⅳ. Genetic and animal models of GI motility disorders caused by loss of interstitial cells of Cajal[J]. Am J Physiol Gastrointest Liver Physiol， 2002， 282(5)：G747-G756.
- 3. Lyford GL， He CL， Soffer E， et al. Pan-colonic decrease in interstitial cells of Cajal in patients with slow transit constipation[J]. Gut， 2002， 51(4)：496-501.
- 4. Wang XY， Vannucchi MG， Nieuwmeyer F， et al. Changes ininterstitial cells of Cajal at the deep muscular plexus are associated with loss of distention induced burst-type muscle activity in mice infected by Trichinella spiralis[J]. Am J Pathol， 2005， 167(2)：437-453.
- 5. Chang IY， Glasgow NJ， Takayama I， et al. Loss of interstitial cells of Cajal and development of electrical dysfunction in murinesmall bowel obstruction[J]. J Physiol， 2001， 536(Pt 2)：555-568.
- 6. Mostafa RM， Moustafa YM， Hamdy H. Interstitial cells of Cajal， the Maestro in health and disease[J]. World J Gastroenterol， 2010， 16(26)：3239-3248.
- 7. Hanani M， Ledder O， Yutkin V， et al. Reeneration of myenteric plexus in the mouse colon after experimental denervation withbenzalkonium chloride[J]. J Comp Neurol， 2003， 462(3)：315-327.
- 8. Yanagida H， Yanase H， Sanders KM， et al. Intestinal surgical resection disrupts electrical rhythmicity， neural responses， and interstitial cells networks[J]. Gastroenterology， 2004， 127(3)：1748-1759.
- 9. Beyder A， Farrugia G. Targeting ion channels for the treatment of gastrointestinal motility disorders[J]. Therap Adv Gastroenterol， 2012， 5(1)：5-21.
- 10. 楼新法， 杨新东， 蒋鹤松， 等. 足三里穴进针角度和深度的研究[J]. 中国针灸， 2006， 26(7)：483-486.
- 11. Blumer-Jensen P， Hunter T. Oncogenic kinase signaling[J]. Nature， 2001， 411(6835)：355-365.
- 12. Huizinga JD， Thuneberg L， Kluppel M， et al. W/kit generequired for interstitial cells of Cajal and for intestinal pacemaker activity[J]. Nature， 1995， 373(6512)： 347-349.
- 13. Chang IY， Glasgow NJ， TakayamaⅠ， et al. Loss of interstitial cells of Cajal and development of electrical dysfunction in murine small bowel obstruction[J]. J Physiol， 2001， 536(2)：555-568.
- 14. Sanders KM. Interstitial cells of Cajal at the clinical and scientific interface[J]. J Physiol， 2006， 576(3)：683-687.
- 15. Won KJ， Suzuki T， Hori M， et al. Motility disorder in experimentally obstructed intestine：relationship between muscularis inflammation and disruption of the ICC network[J]. Neurogastroenterol Motil， 2006， 18(1)：53-61.
- 16. Yanagida H， Sanders KM， Ward SM. Inactivation of inducible nitric oxide synthase protects intestinal pacemaker cells from postoperative damage[J]. J Physiol， 2007， 582(Pt 2)：755-765.
- 17. Yanagida H， Yanase H， Sanders KM. Intestinal surgical resection disrupts electrical rhythmicity， neural responses， and interstitial cells networks[J]. Gastroenterology， 2004， 127(6)：1748-1759.
- 18. Farrugia G. Interstitial cells of Cajal in health and disease[J]. Neurogastroenterol Motil， 2008， 20(Suppl 1)：54-63.
