To investigate the effect of modified cold preservation on rat livers. The modified method was that the vascular bed of rat liver was infused with an additional 20 ml, 30 ml and 40 ml self-made HYD solution/100g liver. Via portal vein and the vesselses of liver were ligated. Forty rats were randomly divided into four groups including conventional storage group (control group) and modified preservation groups (20%, 30% and 40% group). The indices of hepatic microcirculation, enzymes level in the effluent and bile production were determined in the groups. Results: Bile production and all the indices of hepatic microcirculation including portal perfused pressure, endothelin-1 in the effluent, Trypan Blue distrubution time and histology in experimental groups were significantly superior to those in control group (Ｐ＜0.05). The liver enzymes in 30% group were markedly lower than those in control group (Ｐ＜0.05). The preservation effect of rat livers in 30% group was the best among the experimental groups. Conclusions: The modified cold storage method is effective and may have potential for clinical application for liver preservation.
Citation：
SunBei,JiangHongchi,QiaoHaiquan,etal.. STUDYONMODIFIEDCOLDSTORAGEMETHODOFRATLIVERSWITHSELFMADEHYDSOLUTION. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 1999, 6(4): 199-200下转229. doi:
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Copyright © the editorial department of CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY of West China Medical Publisher. All rights reserved
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- 1. LissoniP,BamiS,RovelliF,etal.Thebiologicalsignificanceofsolubleinterleukin2receptorinsolidtumors.EurJCancer,1990;26(1)∶332 LeeWJ,ChangKJ,LeeCS,etal.SelectdepressionofTlymphocytesubsetsingastriccancerpatients:animplicationofimmunotherapy.JSurgOncol,1994;55(3)∶1653 ZengZC,TangZY,LiuKD,etal.ObservationofchangesinperipheralTlymphocytesubsetsbyflowcytometryinpatientswithlivercancertreatedwithradioimmunotherapy.NucMedCommun,1995;16(5)∶3784 RebinLA,Nelson.Thesolubleinterleukin2receptor:biology,functionandclinicalapplication.AnnInternMed,1990;113(8)∶6195 ButchAW,ChungCH.Cytokineexpressionbygerminalcentercells.JImmunol,1993;150(1)∶396 LotzeMT,FinnOJ.Recentadvanceincellularimmunology:implicationsforimmunitytocancer.ImmunolToday,1990;11(4)∶1907 石景森,周连锁,王作仁等.830例肝外胆管癌的回顾性研究.中华外科杂志,1997;35(11)∶6458 Rabeneck.Gallstoneandbileductcancer.Gastroenterology,1991;107(10)∶12059 HensorDE,AlboresSavedraJ,CorleD.Carcinomaofthegallbladder:histologic;stageofdisease,gradeandsurvivedrates.Cancer,1992;70(11)∶1483.
