Objective To explore the feasibility of recombinant adeno-associated virus (rAAV) as a vector for the gene therapy of liver cancer.
Methods The rAAV/enhance green fluorescein protein (EGFP) recombinant was prepared by the routine method of two plasmids cotransfection.
Results The experiment showed that one 10cm plate could produce 107-108 infection unit recombinant by the method of two plasmids cotransfection, and the transduction of HepG2 cell was increased with the increase of infection dosage of rAAV. About 100 multiplicity of infection (MOI) AAV vector could make all the tumor cell light. Conclusion Liver cancer cell can be efficiently transduced by rAAV, and AAV vector may be a valuable vector for the gene therapy of liver cancer.
Citation：
LIU Ziming,YAN Lnan,WANG Zhong,et al.. THE RESEARCH ON THE RECOMBINANT ADENO-ASSOCIATED VIRUS AS A VECTOR FOR THE GENE THERAPY OF LIVER CANCER. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2001, 8(3): 133-134. doi:
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- 1. Koeberl DD, Alxander IE, Halbert CL, et al. Persistent expression of human clotting factor IX from mouse liver after intravenous injection of adenoassociated virus vector 〔J〕. Proc Natl Acad Sci USA, 1997； 94(4)∶1426.
- 2. Xiao X, Juan L, Samulski RJ. Efficient longterm gene transfer into muscle tissue of immunocompetent mice by adenoassociated virus vector 〔J〕. J Virology, 1996； 70(11)∶8098.
- 3. Maass G, Bogedain C, Scheer U, et al. Recombinant adenoassociated virus for the generation of autologous, genemodified tumor vaccine:evidence for a high transduction efficiency into primary epithelial cancer cells 〔J〕. Human Gene Therapy, 1998； 9(7)∶1049.
- 4. Flotte TR, Carter BJ. Adenoassociated virus vectors for gene therapy 〔J〕. Gene therapy, 1995； 2(6)∶357.
