;ObjectiveUsing human tumor necrosis factoralpha (TNFα) genetransduced human liver cancer cell BEL7404 as tumor vaccine, to study the effect of immune rejection to mice liver cancer implanted tumors. MethodsMice were divided into five groups, and were inoculated with TNFα genetransduced BEL7404 cells which irradiated with 60Co (BEL7404TNFCo group), TNFα genetransduced BEL7404 cells (BEL7404TNF group), BEL7404 cells (BEL7404 group), BEL7404 cell irradiated with 60Co (BEL7404Co group) respectively. Normal saline was injected in control group. Then mice liver cancer H22 cells were implanted to each group, the growth of mice liver cancer implanted tumors was observed. The apoptosis index of implanted tumors was detected by TUNEL method.ResultsCompared to BEL7404 group,BEL7404Co group and control group, the tumor vaccine which did not transduce with TNFα gene and the control group, the tumorigenesis rate of liver cancer implanted tumors was reduced, the growth of implanted tumors was inhibited and the apoptosis of implanted tumors was increased in BEL7404TNFCo group,P＜0.01.There was no difference between BEL7404TNFCo group and BEL7404TNF group,P gt;0.05. ConclusionHuman tumor necrosis factoralpha genetransduced human liver cancer cell can be used as tumor vaccine, it has quite b effect of immune rejection to mice liver cancer implanted tumors.
Citation：
LU Yunfei,QIN Xingan,ZENG Jian,LIN Jinling,LIAO Qinghua. Effect of Tumor Necrosis Factoralpha Genetransduced Tumor Vaccine in Mice Liver Cancer Implanted Tumors. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(4): 366-369. doi:
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- 2. 陆云飞，林进令，邱庆明，等. 原发性肝癌基因治疗研究含人TNF基因重组逆转录病毒转移系统的构建与表达 [Ｊ]. 广西医科大学学报， 1997； 14（4）∶1.
- 3. 邱庆明，陆云飞. 转基因人肝癌细胞株（BEL7404/TNF）的建立及其生物活性的初步研究 [Ｊ]. 广西医科大学学报， 1999； 16（4）∶408.
- 4. Wright P, Braun R, Babiuk L, et al. Adenovirusmediated TNFalpha gene transfer induces significant tumor regression in mice [Ｊ]. Cancer Biother Radiopharm, 1999; 14(1)∶49.
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- 6. Koshita Y, Lu Y, Fujii S, et al. Efficacy of TNFalpha genetransduced tumor cells in treatment of established in vivo tumor [Ｊ]. Int J Cancer, 1995; 63(1)∶130.
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