Experiment on Acute Lung Injury in Sprague-Dawley Rats Induced by Organophosphorus Pesticide_West China Medical Journal

Authors：

 CUIYan ,  CAOXiao-ping

Department of Emergency, Affiliated Hospital of Sichuan North Medical College, Nanchong, Sichuan 637000, P. R. China;

Corresponding author：

CAOXiao-ping, Email: cxp120@126.com

Keywords：

Organophosphorus pesticide; Acute lung injury; Nuclear factor-κB; Rats

DOI：

10.7507/1002-0179.20140313

Video：

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Objective To explore the mechanism of lung injury in Sprague-Dawley (SD) rats induced by acute organic phosphorus pesticides (AOPP) by observing the changes of the blood serum nuclear factor (NF)-κB consistence, NF-κB level of lung tissue and lung coefficient. Method Ninety-six healthy male SD rats (six weeks old) were randomly divided into group A (control, n=48) and group B (poison, n=48). The rats of group B were given omethoate by gavage (45 mg/kg), and the rats of group A accepted normal saline. Then the rats were killed at designated observing points (30 minutes; 3, 6, 12, 24, and 48 hours), and the lung coefficient, blood serum NF-κB consistence and NF-κB level of lung tissue were measured. At the same time, we observed the pathological changes of the rats' lung tissue. Results Compared with group A, blood serum NF-κB consistence, NF-κB level of lung tissue and the level of lung coefficient in group B were significantly higher (P<0.01). The lung tissues of group A were normal at each time point, but in group B, the lung pathological changes gradually appeared 30 minutes later with pulmonary interstitial engorging, alveolar septum widening and some alveolus being full of red blood cells, and this situation reached its peak at hour 12. Then it gradually mitigated from 24 to 48 hours. Conclusion There are significant increases in blood serum NF-κB consistence and NF-κB level in lung tissues in rats with lung injury induced by omethoate poisoning. The NF-κB may play a role in the process of lung injury induced by organophosphorus pesticide.

Citation： CUIYan, CAOXiao-ping. Experiment on Acute Lung Injury in Sprague-Dawley Rats Induced by Organophosphorus Pesticide. West China Medical Journal, 2014, 29(6): 1025-1028. doi: 10.7507/1002-0179.20140313 Copy

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1. 李艳辉, 宋德彪, 胡家昌, 等. 急性有机磷农药中毒致呼吸衰竭相关临床因素的探讨[J]. 中国急救医学, 2007, 27(6): 489-491.
2. Semenzato G. Tumour necrosis factor: a cytokine with multiple biological activities[J]. Br J Cancer, 1990, 61(3): 354-361.
3. Eddleston M. The pathophysiology of organophosphorus pesticide self-poisoning is not so simple[J]. Neth J Med, 2008, 66(4): 146-148.
4. Lüscher TF, Wenzel RR, Noll G. Local regulation of the coronary circulation in health and disease[J]. Eur Heart J, 1995, 16(Suppl C): 51-58.
5. 李孟秦, 蒋智, 曹小平. 氧化乐果中毒致肺损伤大鼠血清TNF-α, 肺组织TNF-α表达相关性的实验研究[J]. 当代医学, 2011, 17(21): 44-45.
6. 涂艳阳, 王伯良, 付建芳, 等. TNF-α和TGF-β1在急性有机磷中毒中的临床意义[J]. 细胞与分子免疫学杂志, 2009, 25(12): 1164-1166.
7. Ansaldi D, Hod EA, Stellari F, et al. Imaging pulmonary NF-kappaB activation and therapeutic effects of MLN120B and TDZD-8[J]. PLoS One, 2011, 6(9): e25093.
8. 曹小平, 曹钰. 急诊医学[M]. 北京: 科学出版社, 2014: 299-310.
9. Goswamy A, Ghaudhzoi A. Study of respiratory failure in organophosphate and cariamate poisoning[J]. Heart Lung, 1994, 23(4): 66-72.
10. 贾卫滨, 张海生. 目前救治急性有机磷农药中毒应注意的几个问题[J]. 中华内科杂志, 2000, 39(4): 64-66.
11. Wan F, Lenardo MJ. The nuclear signaling of NF-kappa B: current knowledge, new insights, and future perspectives[J]. Cell Res, 2010, 20(1): 24-33.
12. Liu L, Qiu HB, Yang Y, et al. Losartan, an antagonist of AT1 receptor for angiotensinⅡ, attenuates lipopolysaccharide-induced acute lung injury in rat[J]. Arch Biochem Biophys, 2009, 481(1): 131-136.

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Experiment on Acute Lung Injury in Sprague-Dawley Rats Induced by Organophosphorus Pesticide

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