Monitoring of retinal microcirculation in rabbit by laser speckle imaging technique_Chinese Journal of Ocular Fundus Diseases

Authors：

WuJianguo , ZhengYuqiang , XuHui , 孙静 ,  何伟

He Eye Hospital of He University, Shenyang 110034, China;

Corresponding author：

何伟, Email: 18742471251@163.com

Keywords：

Retinal vein occlusion/physiopathology; Microcirculation; Retinal Vessels/physiopathology

DOI：

10.3760/cma.j.issn.1005-1015.2014.05.016

Video：

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Objective To observe the retinal microcirculation changes in chinchilla rabbit with branch retinal vein occlusion (BRVO), and to evaluate the feasibility of laser speckle imaging (LSI) technology as monitoring tool for retinal microcirculation. Methods Ten 4-month-old chinchilla rabbits were used for the experiment, selecting the right eye as the experimental eye. The main retinal vein, adjacent 0.5-1.0 mm to the optic of rabbit retina, was selected to the target vessel under surgical microscope. The software of LSI instrument was used to measure the diameter of target vein and blood flow of 0.2 mm² area of target vein. The BRVO rabbit model was induced by photodynamic therapy, then measure the diameter and blood flow in the same region using the method as before and after 10 minutes modeled. Results The retinal color pictures, infrared laser and the distribution of blood flow pseudo-color were synchronous displayed by LSI technology. Before and after modeling, the target vessel diameter were (0.104±0.009), (0.128±0.008) mm, and the 0.2 mm² area blood flow of target vessel were (563.500±28.788), (256.000±53.319) PU. The diameter of target blood vessel after modeling was significantly thicker than before, with the significant difference (t=12.14,P=0.008). The blood flow in 0.2 mm² area of target vessel was significantly lower than before, also with the significant difference (t=183.00,P=0.009). Conclusions The diameter of target vessel of the BRVO rabbit model is enlarged, and the target vessel area of 0.2 mm² blood flow is reduced significantly. LSI system can monitor the retinal microcirculation real-time and quantitatively.

Citation： WuJianguo, ZhengYuqiang, XuHui. Monitoring of retinal microcirculation in rabbit by laser speckle imaging technique. Chinese Journal of Ocular Fundus Diseases, 2014, 30(5): 492-494. doi: 10.3760/cma.j.issn.1005-1015.2014.05.016 Copy

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1. Wu J, Zhou X, Hu Y,et al. Video microscope recording of the dynamic course of thrombosis and thrombolysis of the retinal vein in rabbits[J]. Retina, 2010, 30:976-980.
2. Furie B, Furie BC. Mechanisms of thrombus formation[J]. N Engl J Med, 2008, 359:938-949.
3. Ives SJ, Fadel PJ, Brothers RM, et al. Exploring the vascular smooth muscle receptor landscape in vivo:ultrasound doppler versus near-infrared spectroscopy assessments[J]. Am J Physiol Heart Circ Physiol, 2014, 306:771-776.
4. 魏文斌, 杨丽红. 荧光素眼底血管造影的临床应用[J]. 眼科研究, 2006, 31:1-4.
5. 孔平,杨晖,郑刚,等.激光散斑血流成像技术研究新进展[J]. 光学技术, 2014, 40:21-26.
6. Wu J, Zhou X, Sun G, et al. Disposable sutureless silicone contact lens ring for use with a self-sealing cannula system during vitrectomy[J]. Retina, 2010, 30:705-707.
7. 吴建国,李筱荣,汪建涛,等. 用于显微镜下观察眼底和协助手术的动物角膜接触镜:中国, 2009200957135. 2009-12-23.
8. 周小煦, 吴建国. 视网膜静脉阻塞动物模型的制作[J]. 国际眼科杂志, 2006, 6:1109-1112.

Chinese Journal of Ocular Fundus Diseases

Monitoring of retinal microcirculation in rabbit by laser speckle imaging technique

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